LOCUS       40924_16280        7315 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Plant expression vector pDuExP (pDuExAn6), complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7315)
  AUTHORS   Fujikawa Y, Kato N.
  TITLE     Split luciferase complementation assay to study protein-protein 
            interactions in Arabidopsis protoplasts
  JOURNAL   Plant J. 52 (1), 185-195 (2007)
  PUBMED    17662028
REFERENCE   2  (bases 1 to 7315)
  AUTHORS   Kato N.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-APR-2007) Dapartment of Biological Sciences, Louisiana
            State University, 226 Life Sciences Building, Baton Rouge, LA 
            70803-1715, USA
REFERENCE   3  (bases 1 to 7315)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7315)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J."; 
            date: "2007"; volume: "52"; issue: "1"; pages: "185-195"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (19-APR-2007) Dapartment of Biological Sciences, Louisiana State 
            University, 226 Life Sciences Building, Baton Rouge, LA 70803-1715, 
            USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7315
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             9..26
                     /label=6xHis
                     /note="6xHis affinity tag"
     CDS             27..713
                     /codon_start=1
                     /product="NhRluc"
                     /label=NhRluc
                     /note="N-terminal fragment of the Renilla luciferase
                     protein"
                     /protein_id="ABU49619.1"
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGG"
     misc_feature    714..744
                     /note="2x(Gly-Gly-Gly-Gly-Ser); linker"
     protein_bind    750..874
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        1021..1123
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             1124..1780
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     CDS             2125..2427
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     protein_bind    complement(2471..2595)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      2619..2871
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(2880..2896)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(3290..3308)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3326..3342)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3350..3366)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3374..3404)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3419..3440)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3728..4316)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4490..5347)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5348..5452)
                     /label=AmpR promoter
     rep_origin      complement(5530..5985)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     6126..6142
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        6149..6167
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      6198..6207
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        6221..6239
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(6305..6338)
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        6858..7203
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     misc_feature    7232..7287
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
ORIGIN
        1 ctagaatgca tcatcaccat caccatatgg cttccaaggt gtacgacccc gagcaacgca
       61 aacgcatgat cactgggcct cagtggtggg ctcgctgcaa gcaaatgaac gtgctggact
      121 ccttcatcaa ctactatgat tccgagaagc acgccgagaa cgccgtgatt tttctgcatg
      181 gtaacgctgc ctccagctac ctgtggaggc acgtcgtgcc tcacatcgag cccgtggcta
      241 gatgcatcat ccctgatctg atcggaatgg gtaagtccgg caagagcggg aatggctcat
      301 atcgcctcct ggatcactac aagtacctca ccgcttggtt cgagctgctg aaccttccaa
      361 agaaaatcat ctttgtgggc cacgactggg gggcttgtct ggcctttcac tactcctacg
      421 agcaccaaga caagatcaag gccatcgtcc atgctgagag tgtcgtggac gtgatcgagt
      481 cctgggacga gtggcctgac atcgaggagg atatcgccct gatcaagagc gaagagggcg
      541 agaaaatggt gcttgagaat aacttcttcg tcgagaccat gctcccaagc aagatcatgc
      601 ggaaactgga gcctgaggag ttcgctgcct acctggagcc attcaaggag aagggcgagg
      661 ttagacggcc taccctctcc tggcctcgcg agatccctct cgttaagggt ggcggtggtg
      721 gcggttcagg cggaggtggc tctctcgaga caagtttgta caaaaaagct gaacgagaaa
      781 cgtaaaatga tataaatatc aatatattaa attagatttt gcataaaaaa cagactacat
      841 aatactgtaa aacacaacat atccagtcac tatggcggcc gctaagttgg cagcatcacc
      901 cgacgcactt tgcgccgaat aaatacctgt gacggaagat cacttcgcag aataaataaa
      961 tcctggtgtc cctgttgata ccgggaagcc ctgggccaac ttttggcgaa aatgagacgt
     1021 tgatcggcac gtaagaggtt ccaactttca ccataatgaa ataagatcac taccgggcgt
     1081 attttttgag ttatcgagat tttcaggagc taaggaagct aaaatggaga aaaaaatcac
     1141 tggatatacc accgttgata tatcccaatg gcatcgtaaa gaacattttg aggcatttca
     1201 gtcagttgct caatgtacct ataaccagac cgttcagctg gatattacgg cctttttaaa
     1261 gaccgtaaag aaaaataagc acaagtttta tccggccttt attcacattc ttgcccgcct
     1321 gatgaatgct catccggaat tccgtatggc aatgaaagac ggtgagctgg tgatatggga
     1381 tagtgttcac ccttgttaca ccgttttcca tgagcaaact gaaacgtttt catcgctctg
     1441 gagtgaatac cacgacgatt tccggcagtt tctacacata tattcgcaag atgtggcgtg
     1501 ttacggtgaa aacctggcct atttccctaa agggtttatt gagaatatgt ttttcgtctc
     1561 agccaatccc tgggtgagtt tcaccagttt tgatttaaac gtggccaata tggacaactt
     1621 cttcgccccc gttttcacca tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc
     1681 gctggcgatt caggttcatc atgccgtctg tgatggcttc catgtcggca gaatgcttaa
     1741 tgaattacaa cagtactgcg atgagtggca gggcggggcg taaacgcgtg gatccggctt
     1801 actaaaagcc agataacagt atgcgtattt gcgcgctgat ttttgcggta taagaatata
     1861 tactgatatg tatacccgaa gtatgtcaaa aagaggtgtg ctatgaagca gcgtattaca
     1921 gtgacagttg acagcgacag ctatcagttg ctcaaggcat atatgatgtc aatatctccg
     1981 gtctggtaag cacaaccatg cagaatgaag cccgtcgtct gcgtgccgaa cgctggaaag
     2041 cggaaaatca ggaagggatg gctgaggtcg cccggtttat tgaaatgaac ggctcttttg
     2101 ctgacgagaa cagggactgg tgaaatgcag tttaaggttt acacctataa aagagagagc
     2161 cgttatcgtc tgtttgtgga tgtacagagt gatattattg acacgcccgg gcgacggatg
     2221 gtgatccccc tggccagtgc acgtctgctg tcagataaag tctcccgtga actttacccg
     2281 gtggtgcata tcggggatga aagctggcgc atgatgacca ccgatatggc cagtgtgccg
     2341 gtctccgtta tcggggaaga agtggctgat ctcagccacc gcgaaaatga catcaaaaac
     2401 gccattaacc tgatgttctg gggaatataa atgtcaggct cccttataca cagccagtct
     2461 gcaggtcgac catagtgact ggatatgttg tgttttacag tattatgtag tctgtttttt
     2521 atgcaaaatc taatttaata tattgatatt tatatcattt tacgtttctc gttcagcttt
     2581 cttgtacaaa gtggtccgag ctcagctcga atttccccga tcgttcaaac atttggcaat
     2641 aaagtttctt aagattgaat cctgttgccg gtcttgcgat gattatcata taatttctgt
     2701 tgaattacgt taagcatgta ataattaaca tgtaatgcat gacgttattt atgagatggg
     2761 tttttatgat tagagtcccg caattataca tttaatacgc gatagaaaac aaaatatagc
     2821 gcgcaaacta ggataaatta tcgcgcgcgg tgtcatctat gttactagat cgggaattca
     2881 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
     2941 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
     3001 ccttcccaac agttgcgcag cctgaatggc gcccgctcct ttcgctttct tcccttcctt
     3061 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     3121 ccgatttagt gctttacggc acctcgaccc caaaaaactt gatttgggtg atggttcacg
     3181 tagtgcagct cgatatcaaa ttacgccccg ccctgaatca ctagtgcggc cgcctgcagg
     3241 tcgaccatat gggagagctc ccaacgcgtt ggatgcatag cttgagtatt ctatagtgtc
     3301 acctaaatag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     3361 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     3421 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     3481 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     3541 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     3601 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     3661 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     3721 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     3781 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     3841 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     3901 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     3961 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     4021 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     4081 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     4141 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     4201 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     4261 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     4321 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     4381 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     4441 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     4501 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     4561 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     4621 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     4681 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     4741 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     4801 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     4861 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     4921 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     4981 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     5041 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     5101 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     5161 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     5221 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     5281 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     5341 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     5401 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     5461 cccgaaaagt gccacctgat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac
     5521 cgcatcagga aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa
     5581 tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat
     5641 agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg
     5701 tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac
     5761 catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta
     5821 aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag
     5881 ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg
     5941 taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtccatt cgccattcag
     6001 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc
     6061 gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg
     6121 acgttgtaaa acgacggcca gtgaattgta atacgactca ctatagggcg aattgggccc
     6181 gacgtcgcat gctcccggcc gccatggccg cgggattctg taatacgact cactataggg
     6241 cgctagctgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     6301 ccgcataact tcgtatagca tacattatac gaagttatca gtcgacaagc ttgcatgcct
     6361 gcaggtcccc agattagcct tttcaatttc agaaagaatg ctaacccaca gatggttaga
     6421 gaggcttacg cagcaggtct catcaagacg atctacccga gcaataatct ccaggaaatc
     6481 aaataccttc ccaagaaggt taaagatgca gtcaaaagat tcaggactaa ctgcatcaag
     6541 aacacagaga aagatatatt tctcaagatc agaagtacta ttccagtatg gacgattcaa
     6601 ggcttgcttc acaaaccaag gcaagtaata gagattggag tctctaaaaa ggtagttccc
     6661 actgaatcaa aggccatgga gtcaaagatt caaatagagg acctaacaga actcgccgta
     6721 aagactggcg aacagttcat acagagtctc ttacgactca atgacaagaa gaaaatcttc
     6781 gtcaacatgg tggagcacga cacacttgtc tactccaaaa atatcaaaga tacagtctca
     6841 gaagaccaaa gggcaattga gacttttcaa caaagggtaa tatccggaaa cctcctcgga
     6901 ttccattgcc cagctatctg tcactttatt gtgaagatag tggaaaagga aggtggctcc
     6961 tacaaatgcc atcattgcga taaaggaaag gccatcgttg aagatgcctc tgccgacagt
     7021 ggtcccaaag atggaccccc acccacgagg agcatcgtgg aaaaagaaga cgttccaacc
     7081 acgtcttcaa agcaagtgga ttgatgtgat atctccactg acgtaaggga tgacgcacaa
     7141 tcccactatc cttcgcaaga cccttcctct atataaggaa gttcatttca tttggagaga
     7201 acacggggga ctctagctag atatttttac aacaattacc aacaacaaca aacaacaaac
     7261 aacattacaa ttactattta caattacagt cgatctgatc aagagacagg atcct
//
